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KMID : 0545120080180111785
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 11 p.1785 ~ p.1788
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Inhibition of Diacylglycerol Acyltransferase by Phenylpyropenes Produced by Penicillium griseofulvum F1959
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Lee Seung-Woong
Rho Mun-Chual
Choi Jung-Ho
Kim Koan-Hoi
Choi Yong-seok
Lee Hyun-Sun
Kim Young-Kook
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Abstract
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Phenylpyropenes A, B, and C, isolated from Penicillium griseofulvum F1959, inhibited DGAT in rat liver microsomes with IC50 values of 78.7±1.6, 21.7±0.2, and 11.04±0.2 mM, respectively. In addition, a kinetic analysis using a Lineweaver-Burk plot revealed that phenylpyropene C was a noncompetitive inhibitor of DGAT. The apparent Michaelis constant (Km) value and inhibition constant (Ki) value were calculated to be 8 mM and 10.4 mM, respectively. Moreover, phenylpyropene C inhibited triglyceride formation in HepG2 cells.
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KEYWORD
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Penicillium griseofulvum F1959, diacylglycerol acyltransferase (DGAT), phenylpyropene, triglyceride, hepG2 cell
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